Journal: iScience

Article Title: Exendin-4 promotes retinal ganglion cell survival and function by inhibiting calcium channels in experimental diabetes

doi: 10.1016/j.isci.2023.107680

Figure Lengend Snippet: Brn3a-labeled RGCs in control, DM and different drug-treated retinas (A1–C3) Fluorescence micrographs from the peripheral, middle and central regions in flat-mount retinas showing Brn3a-labeled surviving RGCs in normal (A1–C1), DM eyes treated with (A3–C3) or without saline (A2–C2). (A4–C7) Fluorescence micrographs from the peripheral, middle and central regions in flat-mounted retinas depicting Brn3a-labeled RGCs in DM eyes treated with Ex-4 (A4–C4), Ex-9-39+Ex-4 (A5–C5), BayK-8644 (A6–C6), or BayK-8644+Ex-4 (A7–C7). Topical administration of Ex-4 increased RGC viability in the peripheral and middle regions of the retinas (A4, B4). Administration of Ex-9-39 (A5, B5) or BayK-8644 (A7, B7) blocked the protective effects of Ex-4 on RGCs. Scale bar: 50 μm.

Article Snippet: Ex-4, nimodipine, Ex-9-39 and BayK-8644 were purchased from MedChemExpress (Monmouth Junction, NJ, USA), while TTX, Ryanodine, W-7 and U-73122 were from Tocris Bioscience (Ellisville, MO, USA).

Techniques: Labeling, Fluorescence, Saline

Journal: iScience

Article Title: Exendin-4 promotes retinal ganglion cell survival and function by inhibiting calcium channels in experimental diabetes

doi: 10.1016/j.isci.2023.107680

Figure Lengend Snippet: Quantitative analysis of the density of surviving RGCs (A–C) Schematic diagrams of flat-mount retinas, illustration of 12 areas of RGC quantification (4 peripheral, 4 middle and 4 central, indicated by green squares). (D–F) Quantitative analysis of the density of surviving RGCs in the peripheral (D), middle (E) and central regions (F) of retinas from control, DM eyes, saline-treated DM eyes, Ex-4-treated DM eyes, DM eyes treated with Ex-4+Ex-9-39, BayK-8644, or Ex-4+BayK-8644. Retina numbers are noted in the bars. Data are presented as mean ± SD, ∗p < 0.05, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 vs. control; †p < 0.05 and †††p < 0.001 vs. DM + Saline by Tukey’s multiple comparisons test after one-way ANOVA.

Article Snippet: Ex-4, nimodipine, Ex-9-39 and BayK-8644 were purchased from MedChemExpress (Monmouth Junction, NJ, USA), while TTX, Ryanodine, W-7 and U-73122 were from Tocris Bioscience (Ellisville, MO, USA).

Techniques: Saline

Journal: Neuron

Article Title: Neuroligin-1 Signaling Controls LTP and NMDA-Receptors by Distinct Molecular Pathways

doi: 10.1016/j.neuron.2019.02.013

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-HA BioLegend Ct# MMS-101P Mouse anti-β-actin Sigma-Aldrich Ct# AC-15 Mouse anti-Neuroligin 1 Synaptic Systems Ct# 129111 IRDye® 800CW Goat anti-Mouse IgG LI-COR Biosciences Ct# 925-32210 IRDye® 680LT Goat anti-Mouse IgG (H LI-COR Biosciences Ct# 925-68020 Alexa 546, mouse Invitrogen Ct# A-21123; RRID: {"type":"entrez-nucleotide","attrs":{"text":"AB141592","term_id":"62162410","term_text":"AB141592"}} AB141592 Bacterial and Virus Strains AAV DJ-EF1-DIO ChETA-eYFP Stanford Virus Core GVVC-AAV-65 AAV DJ-CMV DIO EGFP Stanford Virus Core GVVC-AAV-12 AAV-DJ-Syn-Cre-GFP Stanford Virus Core GVVC-AAV-117 (VSV-G) Ubiq-EGFP-cre Stanford Virus Core GVVC-lenti-11 (VSV-G) Ubiq-EGFP-deleted cre Stanford Virus Core GVVC-lenti-12 Chemicals, Peptides, and Recombinant Proteins Picrotoxin Sigma-Aldrich Ct# P1675 D-AP5 Tocris Ct# 0106 (±)-Bay K 8644 Tocris Ct# 1544 Cytosine β-D-arabinofuranoside (Ara C) Sigma-Aldrich Ct# 1768 Experimental models: Organisms/Strains Nlgn1 cKO (Nlgn1 tm1Sud /J) Jiang et al., 2017 JAX: 023646 Nlgn1234 cKO This paper Nrxn123β cKO (B6;129-Nrxn3 tm2Sud Nrxn1 tm2Sud Nrxn2 tm2Sud /J) Anderson et al., 2013 JAX: 008416 Software and Algorithms MATLAB MathWorks RRID:SCR_001622 AxoGraph AxoGraph RRID:SCR_014284 MetaMorph Molecular Devices RRID:SCR_002368 pCLAMP 10 Molecular Devices RRID:SCR_011323 Open in a separate window KEY RESOURCES TABLE Nlgn1 extracellular domain is sufficient to rescue LTP loss in cells lacking Nlgn’s Nlgn1 intracellular domain is crucial for basal NMDAR-mediated transmission Binding of Nlgn1 to presynaptic β-Nrxn’s is essential for LTP Spine growth during LTP also requires Nlgn1 binding to β-Nrnx’s

Techniques: Recombinant, Software